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PE Anti-Mouse IL-17A Antibody

RD30500F
Reddot Biotech
ApplicationsFlow Cytometry
Product group Antibodies
TargetIl17a
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    PE Anti-Mouse IL-17A Antibody
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    17F3
  • Concentration
    0.2 mg/ml
  • Conjugate
    RPE
  • Gene ID16171
  • Target name
    Il17a
  • Target description
    interleukin 17A
  • Target synonyms
    Ctl; Ctla; Ctla8; Ctla-8; cytotoxic T-lymphocyte-associated antigen 8; Il; IL-; Il17; IL-17; IL-17A; interleukin-17A
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    The 17F3 monoclonal antibody reacts with mouse IL-17A a 15-20 kDa cytokine expressed by Th17 cells, Oe≥Oe T cells, iNKT cells, NK cells, LTi cells, neutrophils, and intestinal Paneth cells. IL-17A has pleiotropic effects in immunoregulation and inflammation. It plays an important role in anti-microbial and chronic inflammation by inducing cytokine and chemokine production, neutrophil influx, and the production of antibacterial peptides but it is also an inflammatory mediator in the development of autoimmune diseases including rheumatoid arthritis, asthma, multiple sclerosis, and psoriasis. The 17F3 antibody has been shown to neutralize IL-37A?in vivo.
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL17 using anti-IL17 antibody (A00421-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17 antigen affinity purified polyclonal antibody (Catalog # A00421-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17 at approximately 27KD. The expected band size for IL17 is at 17KD.
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