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ICC/IF analysis of HepG2 cells using GTX83904 PFKFB4 antibody [1C8].
ICC/IF analysis of HepG2 cells using GTX83904 PFKFB4 antibody [1C8].
ICC/IF analysis of HepG2 cells using GTX83904 PFKFB4 antibody [1C8].

PFKFB4 antibody [1C8]

GTX83904
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetPFKFB4
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Overview

  • Supplier
    GeneTex
  • Product Name
    PFKFB4 antibody [1C8]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000. ICC/IF: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1C8
  • Concentration
    0.9 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5210
  • Target name
    PFKFB4
  • Target description
    6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
  • Target synonyms
    6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4, 6PF-2-K/Fru-2,6-P2ase 4, 6PF-2-K/Fru-2,6-P2ase testis-type isozyme, PFK/FBPase 4, bifunctional enzyme with kinase and biphosphatase activities
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ16877
  • Protein Name
    6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4
  • Scientific Description
    Synthesis and degradation of fructose 2,6-bisphosphate.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PFKFB4 using anti-PFKFB4 antibody (A07028-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PFKFB4 antigen affinity purified polyclonal antibody (Catalog # A07028-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PFKFB4 at approximately 54 kDa. The expected band size for PFKFB4 is at 54 kDa.
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