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Anti-PGE receptor EP2 (PTGER3) Antibody

CAB4057
Assay Genie
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityMouse
TargetPTGER3
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Anti-PGE receptor EP2 (PTGER3) Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID5733
  • Target name
    PTGER3
  • Target description
    prostaglandin E receptor 3
  • Target synonyms
    EP3, EP3-I, EP3-II, EP3-III, EP3-IV, EP3-VI, EP3e, PGE2-R, lnc003875, prostaglandin E2 receptor EP3 subtype, PGE receptor, EP3 subtype, PGE2 receptor EP3 subtype, prostaglandin E receptor 3 (subtype EP3), prostaglandin receptor (PGE-2), prostanoid EP3 receptor
  • Host
    Rabbit
  • Protein IDP43115
  • Protein Name
    Prostaglandin E2 receptor EP3 subtype
  • Scientific Description
    PGE receptor EP2 (PTGER3) Antibody is a premium polyclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for WB, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Mouse,Rat samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100microL format to meet your experimental needs. For optimal performance, store at -20°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Mouse
  • Reactivity Supplier
    Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PTGER3 using anti-PTGER3 antibody (A02548-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGER3 antigen affinity purified polyclonal antibody (Catalog # A02548-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGER3 at approximately 48 kDa. The expected band size for PTGER3 is at 43 kDa.
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