Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 + PGK2 antibody [HL1775] (GTX637427) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 + PGK2 antibody [HL1775] (GTX637427) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637427/GTX637427_T-44802_20221028_WB_M_R_22110201_713.webp "Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 + PGK2 antibody [HL1775] (GTX637427) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![PGK1 + PGK2 antibody [HL1775] detects PGK1 + PGK2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. PGK1 stained by PGK1 + PGK2 antibody [HL1775] (GTX637427) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637427/GTX637427_T-44802_20221118_IHC-P_R_22112219_598.webp "PGK1 + PGK2 antibody [HL1775] detects PGK1 + PGK2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. PGK1 stained by PGK1 + PGK2 antibody [HL1775] (GTX637427) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![PGK1 + PGK2 antibody [HL1775] detects PGK1 + PGK2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. PGK1 stained by PGK1 + PGK2 antibody [HL1775] (GTX637427) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637427/GTX637427_T-44802_20221118_IHC-P_M_22112219_213.webp "PGK1 + PGK2 antibody [HL1775] detects PGK1 + PGK2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. PGK1 stained by PGK1 + PGK2 antibody [HL1775] (GTX637427) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 + PGK2 antibody [HL1775] (GTX637427) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637427/GTX637427_44851_20221216_WB_Z_22122018_610.webp "Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 + PGK2 antibody [HL1775] (GTX637427) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Whole Japanese medaka extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 + PGK2 antibody [HL1775] (GTX637427) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637427/GTX637427_44851_20250815_WB_medaka_25082121_306.webp "Whole Japanese medaka extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 + PGK2 antibody [HL1775] (GTX637427) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 + PGK2 antibody [HL1775] (GTX637427) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
PGK1 + PGK2 antibody [HL1775]
GTX637427
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat, Zebra Fish
TargetPGK1
Overview
- SupplierGeneTex
- Product NamePGK1 + PGK2 antibody [HL1775]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1775
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5230
- Target namePGK1
- Target descriptionphosphoglycerate kinase 1
- Target synonymsHEL-S-68p, MIG10, PGKA, phosphoglycerate kinase 1, PRP 2, cell migration-inducing gene 10 protein, epididymis secretory sperm binding protein Li 68p, primer recognition protein 2
- HostRabbit
- IsotypeIgG
- Protein IDP00558
- Protein NamePhosphoglycerate kinase 1
- Scientific DescriptionThe protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. [provided by RefSeq, Jan 2014]
- ReactivityHuman, Mouse, Rat, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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