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Phb2 Polyclonal Antibody

CAC10684
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPHB2
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Overview

  • Supplier
    Biomatik
  • Product Name
    PHB2 Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC; Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11331
  • Target name
    PHB2
  • Target description
    prohibitin 2
  • Target synonyms
    B cell receptor associated protein 37; BAP; Bap37; BCAP37; B-cell associated protein; B-cell receptor-associated protein BAP37; D-prohibitin; hBAP; p22; PNAS-141; prohibitin-2; REA; repressor of estrogen receptor activity
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99623
  • Protein Name
    Prohibitin-2
  • Scientific Description
    The Phb2 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of REA/PHB2 using anti-REA/PHB2 antibody (A03315-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human HT1080 whole cell lysates, Lane 6: human SW620 whole cell lysates, Lane 7: human U87 whole cell lysates, Lane 8: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-REA/PHB2 antigen affinity purified polyclonal antibody (Catalog # A03315-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for REA/PHB2 at approximately 33KD. The expected band size for REA/PHB2 is at 33KD.
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