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Western blot All lanes: PHB2 antibody at 3ug/ml Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lane 3: MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 34, 30 kDa Observed band size: 34 kDa
Western blot All lanes: PHB2 antibody at 3ug/ml Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lane 3: MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 34, 30 kDa Observed band size: 34 kDa
Western blot All lanes: PHB2 antibody at 3ug/ml Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lane 3: MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 34, 30 kDa Observed band size: 34 kDa

PHB2 Antibody

CSB-PA859937ESR2HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPHB2
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Overview

  • Supplier
    Cusabio
  • Product Name
    PHB2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11331
  • Target name
    PHB2
  • Target description
    prohibitin 2
  • Target synonyms
    B cell receptor associated protein 37; BAP; Bap37; BCAP37; B-cell associated protein; B-cell receptor-associated protein BAP37; D-prohibitin; hBAP; p22; PNAS-141; prohibitin-2; REA; repressor of estrogen receptor activity
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99623
  • Protein Name
    Prohibitin-2
  • Scientific Description
    Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases (By similarity). Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. Probably involved in regulating mitochondrial respiration activity and in aging.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of REA/PHB2 using anti-REA/PHB2 antibody (A03315-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human HT1080 whole cell lysates, Lane 6: human SW620 whole cell lysates, Lane 7: human U87 whole cell lysates, Lane 8: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-REA/PHB2 antigen affinity purified polyclonal antibody (Catalog # A03315-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for REA/PHB2 at approximately 33KD. The expected band size for REA/PHB2 is at 33KD.
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