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Prohibitin 2 / PHB2 Antibody

LS-C668662
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPHB2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    Prohibitin 2 / PHB2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western blot analysis of PHB2 expression in HepG2 (A), A549 (B), mouse hear (C), rat liver (D) whole cell lysates. Immunohistochemical analysis of PHB2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.. IHC (1:50 - 1:200), IHC-P, WB (1:500 - 1:2000) Western blot analysis of PHB2 expression in HepG2 (A), A549 (B), mouse hear (C), rat liver (D) whole cell lysates. Immunohistochemical analysis of PHB2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID11331
  • Target name
    PHB2
  • Target description
    prohibitin 2
  • Target synonyms
    B cell receptor associated protein 37; BAP; Bap37; BCAP37; B-cell associated protein; B-cell receptor-associated protein BAP37; D-prohibitin; hBAP; p22; PNAS-141; prohibitin-2; REA; repressor of estrogen receptor activity
  • Host
    Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of REA/PHB2 using anti-REA/PHB2 antibody (A03315-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human HT1080 whole cell lysates, Lane 6: human SW620 whole cell lysates, Lane 7: human U87 whole cell lysates, Lane 8: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-REA/PHB2 antigen affinity purified polyclonal antibody (Catalog # A03315-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for REA/PHB2 at approximately 33KD. The expected band size for REA/PHB2 is at 33KD.
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