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Western blot analysis of extracts from 293 cells treated with etoposide using WEE1 (Phospho-Ser642) Antibody.The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from 293 cells treated with etoposide using WEE1 (Phospho-Ser642) Antibody.The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from 293 cells treated with etoposide using WEE1 (Phospho-Ser642) Antibody.The lane on the right is treated with the antigen-specific peptide.

Phospho-WEE1 (Ser642) Antibody

CSB-PA062918
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetWEE1
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Overview

  • Supplier
    Cusabio
  • Product Name
    Phospho-WEE1 (Ser642) Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7465
  • Target name
    WEE1
  • Target description
    WEE1 G2 checkpoint kinase
  • Target synonyms
    WEE1A, WEE1hu, wee1-like protein kinase, WEE1 homolog, WEE1+ homolog, protein kinase, wee1A kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30291
  • Protein Name
    Wee1-like protein kinase
  • Scientific Description
    Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on Tyr-15. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on Tyr-15 and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. Watanabe N., EMBO J. 14:1878-1891(1995). Cichutek A., Cytogenet. Cell Genet. 93:277-283(2001). Igarashi M., Nature 353:80-83(1991)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of WEE1 using anti-WEE1 antibody (A01319-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human A431 whole cell lysates, Lane 4: human U2OS whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WEE1 antigen affinity purified polyclonal antibody (Catalog # A01319-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WEE1 at approximately 72KD. The expected band size for WEE1 is at 72KD.
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