
Untreated (–) and treated (+) C2C12 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody (GTX132597) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody
GTX132597
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPIK3R1
Overview
- SupplierGeneTex
- Product NamePI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.5 mg/ml
- ConjugateUnconjugated
- Gene ID5295
- Target namePIK3R1
- Target descriptionphosphoinositide-3-kinase regulatory subunit 1
- Target synonymsAGM7, GRB1, IMD36, p85, p85-ALPHA, p85alpha, phosphatidylinositol 3-kinase regulatory subunit alpha, PI3 kinase-associated p85, PI3-kinase subunit p85-alpha, PI3K regulatory subunit alpha, growth factor receptor bound 1, phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha), phosphatidylinositol 3-kinase-associated p-85 alpha, phosphoinositide-3-kinase regulatory subunit alpha, phosphoinositide-3-kinase, regulatory subunit 1 (alpha), ptdIns-3-kinase regulatory subunit alpha
- HostRabbit
- IsotypeIgG
- Protein IDP27986
- Protein NamePhosphatidylinositol 3-kinase regulatory subunit alpha
- Scientific DescriptionPhosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161









![PI3 kinase p85 alpha antibody [N2C1], Internal immunoprecipitates PI3 kinase p85 alpha protein in IP experiments. IP samples: Jurkat whole cell extract A. Control with 5 μg of preimmune Rabbit IgG B. Immunoprecipitation of PI3 kinase p85 alpha protein by 5 μg PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) 10 % SDS-PAGE The immunoprecipitated PI3 kinase p85 alpha protein was detected by PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]](https://www.genetex.com/upload/website/prouct_img/normal/GTX111068/GTX111068_40912_IP_w_23060500_860.webp)