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PIK3R1 / p85 Alpha Antibody

LS-C814144
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetPIK3R1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PIK3R1 / p85 Alpha Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC, WB (1:1000 - 1:2000,IHC1:100) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5295
  • Target name
    PIK3R1
  • Target description
    phosphoinositide-3-kinase regulatory subunit 1
  • Target synonyms
    AGM7, GRB1, IMD36, p85, p85-ALPHA, p85alpha, phosphatidylinositol 3-kinase regulatory subunit alpha, PI3 kinase-associated p85, PI3-kinase subunit p85-alpha, PI3K regulatory subunit alpha, growth factor receptor bound 1, phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha), phosphatidylinositol 3-kinase-associated p-85 alpha, phosphoinositide-3-kinase regulatory subunit alpha, phosphoinositide-3-kinase, regulatory subunit 1 (alpha), ptdIns-3-kinase regulatory subunit alpha
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PI 3 Kinase p85 alpha/PIK3R1 using anti-PI 3 Kinase p85 alpha/PIK3R1 antibody (A00318-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PI 3 Kinase p85 alpha/PIK3R1 antigen affinity purified polyclonal antibody (Catalog # A00318-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PI 3 Kinase p85 alpha/PIK3R1 at approximately 85 kDa. The expected band size for PI 3 Kinase p85 alpha/PIK3R1 is at 85 kDa.
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