IHC-P analysis of human ovary adenocarcinoma tissue using GTX83887 PIM2 antibody [5D5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
![IHC-P analysis of human kidney tissue using GTX83887 PIM2 antibody [5D5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83887/GTX83887_1974_IHC-P_w_23061420_779.webp "IHC-P analysis of human kidney tissue using GTX83887 PIM2 antibody [5D5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human liver tissue using GTX83887 PIM2 antibody [5D5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83887/GTX83887_1975_IHC-P_w_23061420_416.webp "IHC-P analysis of human liver tissue using GTX83887 PIM2 antibody [5D5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human pancreas tissue using GTX83887 PIM2 antibody [5D5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83887/GTX83887_1976_IHC-P_w_23061420_426.webp "IHC-P analysis of human pancreas tissue using GTX83887 PIM2 antibody [5D5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human prostate tissue using GTX83887 PIM2 antibody [5D5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83887/GTX83887_1977_IHC-P_w_23061420_883.webp "IHC-P analysis of human prostate tissue using GTX83887 PIM2 antibody [5D5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![FACS analysis of Jurkat cells using GTX83887 PIM2 antibody [5D5]. Red : Primary antibody Blue : Negative control antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX83887/GTX83887_217_FACS_w_23061420_997.webp "FACS analysis of Jurkat cells using GTX83887 PIM2 antibody [5D5]. Red : Primary antibody Blue : Negative control antibody")
![FACS analysis of HEK293T cells transfected with either PIM2 plasmid(Red) or empty vector control plasmid(Blue) using GTX83887 PIM2 antibody [5D5].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83887/GTX83887_218_FACS_w_23061420_753.webp "FACS analysis of HEK293T cells transfected with either PIM2 plasmid(Red) or empty vector control plasmid(Blue) using GTX83887 PIM2 antibody [5D5].")
![IP analysis of DDDDK tagged PIM2 overexpressed HEK293T lysate using GTX83887 PIM2 antibody [5D5].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX83887/GTX83887_3382_IP_w_23061420_429.webp "IP analysis of DDDDK tagged PIM2 overexpressed HEK293T lysate using GTX83887 PIM2 antibody [5D5].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate")
![WB analysis of HEK293T cells transfected with PIM2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83887 PIM2 antibody [5D5]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83887/GTX83887_3971_WB_w_23061420_992.webp "WB analysis of HEK293T cells transfected with PIM2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83887 PIM2 antibody [5D5]. Loading : 5 ug per lane")
![WB analysis of various samples using GTX83887 PIM2 antibody [5D5]. Loading : 10 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX83887/GTX83887_3972_WB_w_23061420_743.webp "WB analysis of various samples using GTX83887 PIM2 antibody [5D5]. Loading : 10 ug per lane Dilution : 1:200")
IHC-P analysis of human ovary adenocarcinoma tissue using GTX83887 PIM2 antibody [5D5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
PIM2 antibody [5D5]
GTX83887
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPIM2
Overview
- SupplierGeneTex
- Product NamePIM2 antibody [5D5]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:2000. ICC/IF: 1:100. IHC-P: 1:50. FACS: 1:100. IP: 2ug/500ul. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID5D5
- Concentration0.54 mg/ml
- ConjugateUnconjugated
- Gene ID11040
- Target namePIM2
- Target descriptionPim-2 proto-oncogene, serine/threonine kinase
- Target synonymsserine/threonine-protein kinase pim-2, pim-2 oncogene, pim-2h, proto-oncogene Pim-2 (serine threonine kinase)
- HostMouse
- IsotypeIgG2a
- Protein IDQ9P1W9
- Protein NameSerine/threonine-protein kinase pim-2
- Scientific DescriptionPromotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappaB kinase/NF-kappaB cascade - this process requires phosphorylation of MAP3K8/COT. Prevents apoptosis induced by growth factor withdrawal via inhibition of caspase-3 activation, and via phosphorylation of pro-apoptotic proteins. Inhibits BAD-induced cell death via phosphorylation of BAD. PIM2-mediated cell survival is glucose-dependent but independent of several AKT regulators such as PI3K, HSP-90 and TOR, indicating that PIM2 and PI3K/AKT/TOR function via distinct pathways. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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