IHC-P analysis of human colon tissue using GTX83892 PIM2 antibody [9A5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
![IHC-P analysis of human liver tissue using GTX83892 PIM2 antibody [9A5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83892/GTX83892_1989_IHC-P_w_23061420_272.webp "IHC-P analysis of human liver tissue using GTX83892 PIM2 antibody [9A5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human prostate tissue using GTX83892 PIM2 antibody [9A5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83892/GTX83892_1990_IHC-P_w_23061420_107.webp "IHC-P analysis of human prostate tissue using GTX83892 PIM2 antibody [9A5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![FACS analysis of HeLa cells using GTX83892 PIM2 antibody [9A5]. Red : Primary antibody Blue : Negative control antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX83892/GTX83892_219_FACS_w_23061420_591.webp "FACS analysis of HeLa cells using GTX83892 PIM2 antibody [9A5]. Red : Primary antibody Blue : Negative control antibody")
![FACS analysis of Jurkat cells using GTX83892 PIM2 antibody [9A5]. Red : Primary antibody Blue : Negative control antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX83892/GTX83892_220_FACS_w_23061420_300.webp "FACS analysis of Jurkat cells using GTX83892 PIM2 antibody [9A5]. Red : Primary antibody Blue : Negative control antibody")
![FACS analysis of HEK293T cells transfected with either PIM2 plasmid(Red) or empty vector control plasmid(Blue) using GTX83892 PIM2 antibody [9A5].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83892/GTX83892_221_FACS_w_23061420_303.webp "FACS analysis of HEK293T cells transfected with either PIM2 plasmid(Red) or empty vector control plasmid(Blue) using GTX83892 PIM2 antibody [9A5].")
![IP analysis of DDDDK tagged PIM2 overexpressed HEK293T lysate using GTX83892 PIM2 antibody [9A5].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX83892/GTX83892_3384_IP_w_23061420_319.webp "IP analysis of DDDDK tagged PIM2 overexpressed HEK293T lysate using GTX83892 PIM2 antibody [9A5].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate")
![WB analysis of HEK293T cells transfected with PIM2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83892 PIM2 antibody [9A5]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83892/GTX83892_3974_WB_w_23061420_786.webp "WB analysis of HEK293T cells transfected with PIM2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83892 PIM2 antibody [9A5]. Loading : 5 ug per lane")
![ICC/IF analysis of COS7 cells transiently transfected with PIM2 plasmid using GTX83892 PIM2 antibody [9A5].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83892/GTX83892_865_ICCIF_w_23061420_213.webp "ICC/IF analysis of COS7 cells transiently transfected with PIM2 plasmid using GTX83892 PIM2 antibody [9A5].")
IHC-P analysis of human colon tissue using GTX83892 PIM2 antibody [9A5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
PIM2 antibody [9A5] - Overexpression Validated
GTX83892
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetPIM2
Overview
- SupplierGeneTex
- Product NamePIM2 antibody [9A5] - Overexpression Validated
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID9A5
- Concentration0.87 mg/ml
- ConjugateUnconjugated
- Gene ID11040
- Target namePIM2
- Target descriptionPim-2 proto-oncogene, serine/threonine kinase
- Target synonymspim-2 oncogene; pim-2h; proto-oncogene Pim-2 (serine threonine kinase); serine/threonine-protein kinase pim-2
- HostMouse
- IsotypeIgG2a
- Scientific DescriptionPromotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappaB kinase/NF-kappaB cascade - this process requires phosphorylation of MAP3K8/COT. Prevents apoptosis induced by growth factor withdrawal via inhibition of caspase-3 activation, and via phosphorylation of pro-apoptotic proteins. Inhibits BAD-induced cell death via phosphorylation of BAD. PIM2-mediated cell survival is glucose-dependent but independent of several AKT regulators such as PI3K, HSP-90 and TOR, indicating that PIM2 and PI3K/AKT/TOR function via distinct pathways. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.
- ReactivityHuman
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203
References
- PIM2-mediated phosphorylation of hexokinase 2 is critical for tumor growth and paclitaxel resistance in breast cancer. Yang T et al., 2018 Nov, OncogeneRead more