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ProductsBack/PKD2 / Polycystin 2 Antibody

PKD2 / Polycystin 2 Antibody

LS-C332683
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetPKD2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PKD2 / Polycystin 2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 53kDa/73kDa/98kDa/103kDa/109kDa, while the observed MW by Western blot was 110kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:1000) The predicted MW is 53kDa/73kDa/98kDa/103kDa/109kDa, while the observed MW by Western blot was 110kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5311
  • Target name
    PKD2
  • Target description
    polycystin 2, transient receptor potential cation channel
  • Target synonyms
    APKD2, PC2, PKD4, Pc-2, TRPP2, polycystin-2, autosomal dominant polycystic kidney disease type II protein, polycystic kidney disease 2 (autosomal dominant), transient receptor potential cation channel subfamily P member 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PKD2 using anti-PKD2 antibody (A00630-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: rat spleen tissue lysates, Lane 3: mouse thymus tissue lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse SP20 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKD2 antigen affinity purified polyclonal antibody (Catalog # A00630-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKD2 at approximately 110KD. The expected band size for PKD2 is at 110KD.
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