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Formalin-fixed and paraffin embedded human kidney carcinoma labeled with Anti-Polycystin 2 Polyclonal Antibody, Unconjugated (bs-2158R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human kidney carcinoma labeled with Anti-Polycystin 2 Polyclonal Antibody, Unconjugated (bs-2158R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human kidney carcinoma labeled with Anti-Polycystin 2 Polyclonal Antibody, Unconjugated (bs-2158R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Polycystin 2 Polyclonal Antibody

BS-2158R
Bioss
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Human, Mouse, Rat
TargetPKD2
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Overview

  • Supplier
    Bioss
  • Product Name
    Polycystin 2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5311
  • Target name
    PKD2
  • Target description
    polycystin 2, transient receptor potential cation channel
  • Target synonyms
    APKD2, PC2, PKD4, Pc-2, TRPP2, polycystin-2, autosomal dominant polycystic kidney disease type II protein, polycystic kidney disease 2 (autosomal dominant), transient receptor potential cation channel subfamily P member 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13563
  • Protein Name
    Polycystin-2
  • Reactivity
    Bovine, Canine, Chicken, Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PKD2 using anti-PKD2 antibody (A00630-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: rat spleen tissue lysates, Lane 3: mouse thymus tissue lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse SP20 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKD2 antigen affinity purified polyclonal antibody (Catalog # A00630-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKD2 at approximately 110KD. The expected band size for PKD2 is at 110KD.
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Immunohistochemical staining of human kidney shows cytoplasmic positivity in cells in tubules.
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Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Polycystin 2 antibody (GTX113802) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GeneTex
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