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ProductsBack/Plzf (ZBTB16) Mouse Monoclonal Antibody [Clone ID: 5B3]
Western blot analysis using ZBTB16 mouse mAb against Hela (1) cell lysate.
Western blot analysis using ZBTB16 mouse mAb against Hela (1) cell lysate.
Western blot analysis using ZBTB16 mouse mAb against Hela (1) cell lysate.

Plzf (ZBTB16) Mouse Monoclonal Antibody [Clone ID: 5B3]

AM06484SU-N
OriGene
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetZBTB16
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    Plzf (ZBTB16) Mouse Monoclonal Antibody [Clone ID: 5B3]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5B3
  • Gene ID7704
  • Target name
    ZBTB16
  • Target description
    zinc finger and BTB domain containing 16
  • Target synonyms
    PLZF, ZNF145, zinc finger and BTB domain-containing protein 16, promyelocytic leukaemia zinc finger, zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia), zinc finger protein PLZF
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ05516
  • Protein Name
    Zinc finger and BTB domain-containing protein 16
  • Scientific Description
    Plzf (ZBTB16) mouse monoclonal antibody, clone 5B3, Ascites
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Plzf/ZBTB1 using Plzf/ZBTB1 antibody (A00817-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human Raji whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: rat spleen tissue lysates, Lane 8: mouse Raw264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Plzf/ZBTB1 antigen affinity purified polyclonal antibody (Catalog # A00817-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Plzf/ZBTB1 at approximately 74KD. The expected band size for Plzf/ZBTB1 is at 74KD.
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