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PMAIP1 / NOXA Antibody

LS-C668202
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetPMAIP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PMAIP1 / NOXA Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western blot analysis of NOXA expression in Jurkat (A), Hela (B), SKOV3 (C) whole cell lysates.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) Western blot analysis of NOXA expression in Jurkat (A), Hela (B), SKOV3 (C) whole cell lysates.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5366
  • Target name
    PMAIP1
  • Target description
    phorbol-12-myristate-13-acetate-induced protein 1
  • Target synonyms
    APR, NOXA, phorbol-12-myristate-13-acetate-induced protein 1, PMA-induced protein 1, adult T cell leukemia-derived PMA-responsive, immediate-early-response protein APR, protein Noxa
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PMAIP1 using anti-PMAIP1 antibody (M02287). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PMAIP1 antigen affinity purified monoclonal antibody (Catalog # M02287) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PMAIP1 at approximately 16 kDa. The expected band size for PMAIP1 is at 6 kDa.
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