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Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA018299LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA018299LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA018299LA01HU at dilution of 1:100

POGZ Antibody

CSB-PA018299LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPOGZ
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Overview

  • Supplier
    Cusabio
  • Product Name
    POGZ Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23126
  • Target name
    POGZ
  • Target description
    pogo transposable element derived with ZNF domain
  • Target synonyms
    MRD37; pogo transposable element with ZNF domain; putative protein product of Nbla00003; WHSUS; zinc finger protein 280E; zinc finger protein 635; ZNF280E; ZNF635; ZNF635m
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7Z3K3
  • Protein Name
    Pogo transposable element with ZNF domain
  • Scientific Description
    Plays a role in mitotic cell cycle progression and is involved in kinetochore assembly and mitotic sister chromatid cohesion. Probably through its association with CBX5 plays a role in mitotic chromosome segregation by regulating aurora kinase B/AURKB activation and AURKB and CBX5 dissociation from chromosome arms.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of POGZ using anti-POGZ antibody (A06169-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POGZ antigen affinity purified polyclonal antibody (Catalog # A06169-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POGZ at approximately 220 kDa. The expected band size for POGZ is at 155 kDa.
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