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Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neurons.

Anti-POGZ Antibody

HPA006800
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPOGZ
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-POGZ Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23126
  • Target name
    POGZ
  • Target description
    pogo transposable element derived with ZNF domain
  • Target synonyms
    MRD37, WHSUS, ZNF280E, ZNF635, ZNF635m, pogo transposable element with ZNF domain, putative protein product of Nbla00003, zinc finger protein 280E, zinc finger protein 635
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7Z3K3
  • Protein Name
    Pogo transposable element with ZNF domain
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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THP-1 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Pogz antibody [HL1898] (GTX637647) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Figure 1. Western blot analysis of POGZ using anti-POGZ antibody (A06169-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POGZ antigen affinity purified polyclonal antibody (Catalog # A06169-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POGZ at approximately 220 kDa. The expected band size for POGZ is at 155 kDa.
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