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ProductsBack/Pogz antibody [HL1898]
THP-1 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Pogz antibody [HL1898] (GTX637647) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
THP-1 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Pogz antibody [HL1898] (GTX637647) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
THP-1 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Pogz antibody [HL1898] (GTX637647) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Pogz antibody [HL1898]

GTX637647
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetPOGZ
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Overview

  • Supplier
    GeneTex
  • Product Name
    Pogz antibody [HL1898]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL1898
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID23126
  • Target name
    POGZ
  • Target description
    pogo transposable element derived with ZNF domain
  • Target synonyms
    MRD37, WHSUS, ZNF280E, ZNF635, ZNF635m, pogo transposable element with ZNF domain, putative protein product of Nbla00003, zinc finger protein 280E, zinc finger protein 635
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7Z3K3
  • Protein Name
    Pogo transposable element with ZNF domain
  • Scientific Description
    The protein encoded by this gene appears to be a zinc finger protein containing a transposase domain at the C-terminus. This protein was found to interact with the transcription factor SP1 in a yeast two-hybrid system. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Aug 2010]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of POGZ using anti-POGZ antibody (A06169-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POGZ antigen affinity purified polyclonal antibody (Catalog # A06169-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POGZ at approximately 220 kDa. The expected band size for POGZ is at 155 kDa.
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