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ProductsBack/POGZ Antibody (FITC)

POGZ Antibody (FITC)

LS-C396822
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
TargetPOGZ
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    POGZ Antibody (FITC)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    ELISA
  • Applications Supplier
    ELISA The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    FITC
  • Estimated Purity
    ...
  • Gene ID23126
  • Target name
    POGZ
  • Target description
    pogo transposable element derived with ZNF domain
  • Target synonyms
    MRD37; pogo transposable element with ZNF domain; putative protein product of Nbla00003; WHSUS; zinc finger protein 280E; zinc finger protein 635; ZNF280E; ZNF635; ZNF635m
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of POGZ using anti-POGZ antibody (A06169-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POGZ antigen affinity purified polyclonal antibody (Catalog # A06169-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POGZ at approximately 220 kDa. The expected band size for POGZ is at 155 kDa.
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