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ProductsBack/PP1 antibody [HL1677]
Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with PPP1A antibody [HL1677] (GTX637276) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with PPP1A antibody [HL1677] (GTX637276) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with PPP1A antibody [HL1677] (GTX637276) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

PP1 antibody [HL1677]

GTX637276
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetPPP1CA
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Overview

  • Supplier
    GeneTex
  • Product Name
    PP1 antibody [HL1677]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL1677
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5499
  • Target name
    PPP1CA
  • Target description
    protein phosphatase 1 catalytic subunit alpha
  • Target synonyms
    PP-1A, PP1A, PP1alpha, PPP1A, serine/threonine-protein phosphatase PP1-alpha catalytic subunit, protein phosphatase 1, catalytic subunit, alpha isozyme, serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit, testicular tissue protein Li 155
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP36873
  • Protein Name
    Serine/threonine-protein phosphatase PP1-gamma catalytic subunit
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PPP1CA + PPP1CB using anti-PPP1CA + PPP1CB antibody (M02801). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human U87 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1CA + PPP1CB antigen affinity purified monoclonal antibody (Catalog # M02801) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1CA + PPP1CB at approximately 38 kDa. The expected band size for PPP1CA + PPP1CB is at 38 kDa.
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