![WB analysis of 25 ug of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) using GTX12412 PPAR alpha antibody [3B6/PPAR]. Dilution : 1:1000 (lane 1 and 2) & 1:500 (lane 3) WB analysis of 25 ug of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) using GTX12412 PPAR alpha antibody [3B6/PPAR]. Dilution : 1:1000 (lane 1 and 2) & 1:500 (lane 3)](https://www.genetex.com/upload/website/prouct_img/normal/GTX12412/GTX12412_1480_WB_w_23060522_816.webp)
WB analysis of 25 ug of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) using GTX12412 PPAR alpha antibody [3B6/PPAR]. Dilution : 1:1000 (lane 1 and 2) & 1:500 (lane 3)
PPAR alpha antibody [3B6/PPAR]
GTX12412
ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
Product group Antibodies
TargetPPARA
Overview
- SupplierGeneTex
- Product NamePPAR alpha antibody [3B6/PPAR]
- Delivery Days Customer9
- Application Supplier NoteWB: 2-5 microg/ml. ICC/IF: 2microg/ml. FACS: 1microg per 106 cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID3B6/PPAR
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5465
- Target namePPARA
- Target descriptionperoxisome proliferator activated receptor alpha
- Target synonymsNR1C1, PPAR, PPAR-alpha, PPARalpha, hPPAR, peroxisome proliferator-activated receptor alpha, nuclear receptor subfamily 1 group C member 1, peroxisome proliferative activated receptor, alpha
- HostMouse
- IsotypeIgG2b
- Protein IDQ07869
- Protein NamePeroxisome proliferator-activated receptor alpha
- Scientific DescriptionPeroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq, Jul 2008]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![ICC/IF analysis of HCT116 cells using GTX12412 PPAR alpha antibody [3B6/PPAR]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Trito X-100 for 10 minutes Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature ICC/IF analysis of HCT116 cells using GTX12412 PPAR alpha antibody [3B6/PPAR]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Trito X-100 for 10 minutes Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX12412/GTX12412_246_ICC-IF_w_23060522_766.webp)
![FACS analysis of 3T3 cells(1-5x10? cells/ml) using GTX12412 PPAR alpha antibody [3B6/PPAR] compared to an isotype control (blue). Dilution : 0.25 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde FACS analysis of 3T3 cells(1-5x10? cells/ml) using GTX12412 PPAR alpha antibody [3B6/PPAR] compared to an isotype control (blue). Dilution : 0.25 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde](https://www.genetex.com/upload/website/prouct_img/normal/GTX12412/GTX12412_88_FACS_w_23060522_280.webp)
![FACS analysis of Hela cells(1-5x10? cells/ml) using GTX12412 PPAR alpha antibody [3B6/PPAR] compared to an isotype control (blue). Dilution : 0.25 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde FACS analysis of Hela cells(1-5x10? cells/ml) using GTX12412 PPAR alpha antibody [3B6/PPAR] compared to an isotype control (blue). Dilution : 0.25 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde](https://www.genetex.com/upload/website/prouct_img/normal/GTX12412/GTX12412_89_FACS_w_23060522_640.webp)
![FACS analysis of Jurkat cells(1-5x10? cells/ml) using GTX12412 PPAR alpha antibody [3B6/PPAR] compared to an isotype control (blue). Dilution : 0.25 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde FACS analysis of Jurkat cells(1-5x10? cells/ml) using GTX12412 PPAR alpha antibody [3B6/PPAR] compared to an isotype control (blue). Dilution : 0.25 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde](https://www.genetex.com/upload/website/prouct_img/normal/GTX12412/GTX12412_90_FACS_w_23060522_233.webp)

![FACS analysis of 3T3 cells using GTX12412 PPAR alpha antibody [3B6/PPAR] compared to an isotype control (blue). Dilution : 0.25 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde](https://www.genetex.com/upload/website/prouct_img/normal/GTX22779/GTX22779_138_FACS_w_23060620_338.webp)


