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WB analysis of HepG2 cells using GTX45142 PRAME antibody at 0.2-1microg/ml.
WB analysis of HepG2 cells using GTX45142 PRAME antibody at 0.2-1microg/ml.
WB analysis of HepG2 cells using GTX45142 PRAME antibody at 0.2-1microg/ml.

PRAME antibody, N-term

GTX45142
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetPRAME
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Overview

  • Supplier
    GeneTex
  • Product Name
    PRAME antibody, N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID23532
  • Target name
    PRAME
  • Target description
    PRAME nuclear receptor transcriptional regulator
  • Target synonyms
    cancer/testis antigen 130; CT130; MAPE; melanoma antigen preferentially expressed in tumors; OIP4; OIP-4; opa-interacting protein 4; Opa-interacting protein OIP4; preferentially expressed antigen in melanoma; preferentially expressed antigen of melanoma
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP78395
  • Protein Name
    Melanoma antigen preferentially expressed in tumors
  • Scientific Description
    This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PRAME using anti-PRAME antibody (A06628-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRAME antigen affinity purified polyclonal antibody (Catalog # A06628-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRAME at approximately 58KD. The expected band size for PRAME is at 58KD.
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