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Primary Antibodies

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Western blot using GeneTex's purified anti-p47 ING3 antibody shows detection of a band at ~55 kDa corresponding to ING3 in RKO cells transfected with ING3 (lane 2). Control RKO cells do not show detection of this specific band (lane 1). The identity of the non-specific bands at 33 kDa and 20 kDa has not been determined. Each lane contains approximately 10 μg of RKO whole cell lysate separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose. After blocking with 5% NF dry milk, the membrane was probed with the primary antibody diluted to 1:1,000. Incubation was at 4o C overnight followed by washes and reaction with a 1:20,000 dilution of IRDye?800 conjugated Rb-a-Goat IgG [H&L] MXHur for 45 min at room temperature. IRDye?800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Product group Antibodies
GeneTex
ING3 antibodyGTX48488
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetING3
  • SizePrice
Product group Antibodies
GeneTex
AChE antibodyGTX48490
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityBovine
TargetACHE
  • SizePrice
Western blot using GeneTex's Affinity Purified anti-POLK antibody shows detection of a band ~98 kDa corresponding to human POLK (arrowhead). Approximately 35 μg of a HeLa whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4o followed by washes and reaction with a 1:20,000 dilution of IRDye800 conjugated rabbit anti-Goat IgG [H&L] MXHu for 45 min at room temperature. IRDye800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Product group Antibodies
GeneTex
POLK antibodyGTX48495
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetPOLK
  • SizePrice
Western blot shows detection of recombinant NAG-1 protein (arrow) present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag at 36 kDa; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag at 36 kDa; lane 3 - yeast cell lysate expressing NAG-1 H variant; and lane 4 - yeast cell lysate expressing NAG-1 D variant. All lysates were run under reducing conditions. Primary antibody was used at a 1:1000 dilution in TBS containing 1% BSA and 0.2% Tween, and reacted overnight at 4oC. For detection, a 1:40,000 dilution of peroxidase conjugated Goat anti-Mouse IgG secondary antibody for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers.
Product group Antibodies
GeneTex
GDF15 antibody [23G10.F8]GTX48497
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetGDF15
  • SizePrice
Western blot using GeneTex's anti-NAG-1 monoclonal antibody. The blot shows detection of recombinant NAG-1 protein present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag at 36 kDa; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag at 36 kDa; lane 3 - yeast cell lysate expressing NAG-1 H variant; and lane 4 - yeast cell lysate expressing NAG-1 D variant. Recombinant NAG-1 proteins without SUMO correspond to monomer (15 kDa) and dimer (30 kDa) bands as indicated by the arrowheads. All lysates were run under reducing conditions. Primary antibody was used at a 1:1,000 dilution in TBS containg 1% BSA and 0.2% Tween, and reacted overnight at 4oC. For detection, a 1:40,000 dilution of peroxidase conjugated Goat-anti-Mouse IgG secondary antibody for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers.
Product group Antibodies
GeneTex
GDF15 antibody [23B3D2.H5]GTX48498
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetGDF15
  • SizePrice
Western blot shows detection of recombinant NAG-1 protein present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag at 36 kDa; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag at 36 kDa; lane 3 - yeast cell lysate expressing NAG-1 H variant; and lane 4 - yeast cell lysate expressing NAG-1 D variant. All lysates were run under reducing conditions. Primary antibody was used at a 1:1,000 dilution in TBS containing 1% BSA and 0.2% Tween, and reacted overnight at 4oC. For detection, a 1:40,000 dilution of peroxidase conjugated Goat-anti-Mouse IgG secondary antibody for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers.
Product group Antibodies
GeneTex
GDF15 (H Variant) antibody [7C12.B3.F2]GTX48499
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetGDF15
  • SizePrice
Product group Antibodies
GeneTex
Hand2 antibody, C-termGTX48501
ApplicationsELISA
ReactivityZebra Fish
Targethand2
  • SizePrice
Product group Antibodies
GeneTex
Merlin antibody, InternalGTX48502
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityZebra Fish
Targetnf2b
  • SizePrice
Product group Antibodies
References
GeneTex
Notch1a antibody, InternalGTX48504
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityZebra Fish
Targetnotch1a
  • SizePrice
Product group Antibodies
GeneTex
Patched 1 antibody, InternalGTX48506
ApplicationsImmunoHistoChemistry
ReactivityZebra Fish
Targetptch1
  • SizePrice
Product group Antibodies
GeneTex
Sox9b antibody, InternalGTX48509
ApplicationsELISA
ReactivityZebra Fish
Targetsox9b
  • SizePrice
WB analysis of zebrafish adult heart lysate using GTX48511 Thrb antibody, Internal. Dilution : 2.5μg/ml Loading : 35μg protein in RIPA buffer
Product group Antibodies
GeneTex
Thrb antibody, InternalGTX48511
ApplicationsWestern Blot
ReactivityZebra Fish
Targetthrb
  • SizePrice
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