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Phosphospecificity Whole cell extracts of Control (Lane 1) or Insulin stimulated (Lane 2) MDA-MB-213 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The Immunoblot was probed with Monoclonal InsR antibody 10C3 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetINSR
  • SizePrice
Phosphospecificity: Whole cell extracts of control (co) or Insulin, IGF1, Pervanadate stimulated MDA-MB-231 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to PVDF membranes. Immunoblots were probed with Monoclonal InsR antibody 21G12 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: untreated Lane 2: Insulin-treated Lane 3: IGF1-treated Lane 4: Pervanadate-treated
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse
TargetINSR
  • SizePrice
Detection of endogenous InsR: Whole cell extracts of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab InsR-9H4 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 3 min). Lane 1: A431 Lane 2: SW480 Lane 3: SW620 Lane 4: HT29 Lane 5: MCF-7 Lane 6: MDA-MB231 Lane 7: T47D
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse, Rat
TargetINSR
  • SizePrice
Detection of endogenous InsR: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab InsR-11B6 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: HeLa; lane 2: HepG2; lane 3: HEK293; lane 4: SH-SY5Y; lane 5: MDCK; lane 6: PC12; lane 7: CMT 93; lane 8: Neuro 2A
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
TargetINSR
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse, Rat
TargetMAPK9
  • SizePrice
Figure 1. Detection of endogenous SAPK1alpha Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab SAPK1a/jnk2-12C5 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse, Rat
TargetMAPK9
  • SizePrice
Detection of endogenous SAPK1 beta Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab SAPK1b/jnk3-4G6 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman
TargetMAPK10
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse, Rat
TargetMAPK8
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse, Rat
TargetMAPK8
  • SizePrice
Immunoblot Analysis Jurkat cells were cultured under serum-free conditions for 48h and subsequently were either unstimulated (lane 1) or stimulated with 300mM sorbit (lane 2), 1mM arsenite (lane 3) or 1mM pervanadate (lane 4).The cell lysates were separated by SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab SAPK1/2-9H8 at 0.5ug/ml for 1h at 15-22°C and developed by ECL.
Product group Antibodies
OriGene
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetMAPK8
  • SizePrice
Detection of endogenous LEF Whole cell lysates of serum starved Jurkat were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab 2D12 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetLEF1
  • SizePrice
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse, Rat
TargetMAPK3
  • SizePrice

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