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Phosphospecificity Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) A431 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to PVDF membranes. Immunoblots were probed with mab GSK-9B8 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
TargetGSK3A
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
TargetGSK3B
  • SizePrice
Detection of Endogenous GSK3 beta: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab GSK3-11B9 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2:SW480 Lane 3: SW620 Lane 4: HT29 Lane 5: MCF7 Lane 6: MDA-MB-231 Lane 7: T47D
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
TargetGSK3B
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse
TargetGSK3B
  • SizePrice
Phosphospecificity Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) A431 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to PVDF membranes. Immunoblots were probed with mab GSK-3A8 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse
TargetGSK3B
  • SizePrice
Phosphospecificity Whole cell extracts of control (co) and pervanadate (VH) treated A431 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to PVDF membranes. Immunoblots were probed with mab GSK3b-2D3 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
TargetGSK3B
  • SizePrice
Antibody sensitivity Recombinant GST-PIN1 was separated by SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab 4H3 (0.5 g/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: 100ng, lane 2: 50ng, lane 3: 25ng, lane 4
Product group Antibodies
OriGene
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivitySchistosoma japonicum
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse, Rat
TargetHSPB1
  • SizePrice
HSP 27 activation Serum starved HepG2 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). Immunoblots were probed with mab hsp27-5B9 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: Control Lane 2: 5 min EGF Lane 3: 15 min EGF Lane 4: 30 min EGF Lane 5: 1h EGF Lane 6; 2h EGF Lane 7: 4h EGF Lane 8: 8h EGF
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse, Rat
TargetHSPB1
  • SizePrice
Phosphospecificity Whole cell extracts of control (co) or Insulin, IGF1 stimulated HeLa tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to PVDF membranes. Immunoblots were probed with mab IGF1R-2B9 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: Co; lane 2:Insulin; lane 3: IGF1
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetIGF1R
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman
TargetIGF1R
  • SizePrice
Detection of endogenous IGF1R Whole cell extracts of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab IGF1R-7G11 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman
TargetIGF1R
  • SizePrice

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