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Product group Antibodies
OriGene
ErbB 3 (ERBB3) (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11A4]AM00054BT-N
ApplicationsWestern Blot
ReactivityHuman
TargetERBB3
  • SizePrice
Detection of Endogenous ErbB3: Whole cell lysates of EGF stimulated serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab erbB3-11A4 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: MDA-MB 231 Lane 2: MDA-MB 468 Lane 3: MCF-7 Lane 4: T47D Lane 5: SW480 Lane 6: SW620
Product group Antibodies
OriGene
ErbB 3 (ERBB3) (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11A4]AM00054PU-N
ApplicationsWestern Blot
ReactivityHuman
TargetERBB3
  • SizePrice
Detection of endogenous erbB3: Whole cell lysates of EGF stimulated serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab erbB3-5A12 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: MDA-MB 231; lane 2: MDA-MB 468; lane 3: MCF-7; lane 4: T47D; lane 5: SW480; lane 6: SW620
Product group Antibodies
OriGene
ErbB 3 (ERBB3) (Cytopl. Dom.) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 5A12]AM00055PU-N
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman
TargetERBB3
  • SizePrice
Detection of endogenous erbB4: Whole cell extracts of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab erbB4-6C5 (0.5 ug/ ml) for 1h at 15-22°C and developed by ECL (exp. time: 30 sec). Lane 1: MDA-MB 231 Lane 2: MDA-MB 468 Lane 3: MCF-7 Lane 4: T47D Lane 5: SW480 Lane 6: SW620
Product group Antibodies
OriGene
ErbB 4 (ERBB4) (1230-1250) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 6C5]AM00056PU-N
ApplicationsWestern Blot
ReactivityHuman
TargetERBB4
  • SizePrice
erbB4 activation Serum starved MDA-MB468 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab erbB4 - 4C6 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: control; lane 2: 5 min EGF; lane 3: 15 min EGF; lane 4: 30 min EGF; lane 5: 1h EGF; lane 6: 2h EGF lane 7: 4h EGF; lane 8: 8h EGF
Product group Antibodies
OriGene
ErbB 4 (ERBB4) pTyr1242 (incl. pos. Control) Mouse Monoclonal Antibody [Clone ID: 4C6]AM00057PU-N
ApplicationsWestern Blot
ReactivityHuman
TargetERBB4
  • SizePrice
Detection of endogenous MAPK7 / erk5 Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. Immunoblots were probed with mab MAPK7-12F2 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2: A549 Lane 3: SKOV3 Lane 4: OVCAR5 Lane 5: HaCaT Lane 6: PC3 Lane 7: HeLa Lane 8: HepG2
Product group Antibodies
OriGene
ERK5 (MAPK7) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 12F2]AM00058PU-N
ApplicationsWestern Blot
ReactivityCanine, Human, Mouse, Rat
TargetMAPK7
  • SizePrice
Detection of endogenous FAK: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab AM00059PU-N (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK293 Lane 4: SH-SY5Y Lane 5: MDCK Lane 6: PC12 Lane 7: CMT 93 Lane 8: Neuro 2A Lane 9: 3T3
Product group Antibodies
OriGene
FAK (PTK2) pTyr397 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 2D11]AM00059PU-N
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
TargetPTK2
  • SizePrice
Detection of endogenous fer: Whole cell extracts of pervanadate (VH) treated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab fer-6B9 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). VH treatment: 15 min. Lane 1: A431; Lane 2: SW480 lane 3: SW620; lane 4: HT29; lane 5: MCF-7; lane 6: MDA-MB231; lane 7: T47D
Product group Antibodies
OriGene
FER (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 6B9]AM00060PU-N
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
TargetFER
  • SizePrice
fos activation Serum starved HepG2 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). Immunoblots were probed with mab fos-34E4 (0.5 ug/ ml) for 1h at room temperature and developed by ECL (exp. time: 30 sec). lane 1: control; lane 2: 5 min EGF; lane 3: 15 min EGF; lane 4: 30 min EGF; lane 5: 1h EGF;lane 6; 2h EGF lane 7: 4h EGF; lane 8: 8h EGF
Product group Antibodies
OriGene
c Fos (FOS) pSer374 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 34E4]AM00061PU-N
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityCanine, Human
TargetFOS
  • SizePrice
Detection of endogenous FOS: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab FOS-8B5 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2: A549 Lane 3: SKOV3 Lane 4: OVCAR5 Lane 5: HaCaT Lane 6: PC3 Lane 7: HeLa Lane 8: HepG2
Product group Antibodies
OriGene
c Fos (FOS) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 8B5]AM00062PU-N
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse, Rat
TargetFOS
  • SizePrice
Product group Antibodies
OriGene
N-Acetylglucosamine (GlcNAc) Mouse Monoclonal Antibody [Clone ID: 10D8]AM00063PU-N
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
  • SizePrice
Detection of endogenous GSK3alpha Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab GSK3a-10C1 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2: A549 Lane 3: SKOV-3 Lane 4: OVCAR-5 Lane 5: HaCaT Lane 6: PC3M Lane 7: Hela Lane 8: HepG2
Product group Antibodies
OriGene
GSK3 alpha (GSK3A) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 10C1]AM00064PU-N
ApplicationsWestern Blot
ReactivityCanine, Human, Mouse, Rat
TargetGSK3A
  • SizePrice
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