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IHC image of CSB-RA008642MA1HU diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Rabbit IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetTK1
Overlay Peak curve showing Hela cells stained with CSB-RA023603MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was human IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetTLR4
The Binding Activity of Human TM4SF1 with Anti-TM4SF1 recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human TM4SF1(CSB-MP023615HU) at 5 microg/mL can bind Anti-TM4SF1 recombinant antibody. the EC50 is 4.079-4.472 ng/mL. The VLPs (CSB-MP3838) is negative control.
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetTM4SF1
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, U251 whole cell lysate All lanes: TNFRSF10B antibody at 1:500 Secondary Goat polyclonal to Human IgG at 1/50000 dilution Predicted band size: 13 kDa Observed band size: 13 kDa
Product group Antibodies
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
ReactivityHuman
TargetTNFRSF10B
IHC image of CSB-RA023981MA1HU diluted at 1:200 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetTNFRSF4
Western Blot Positive WB detected in: HT-29 whole cell lysate All lanes: TP53 antibody at 1:500 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 43 kDa Observed band size: 43 kDa
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
ReactivityHuman
TargetTP53
Overlay Peak curve showing MCF7 cells stained with CSB-RA024093MA1HU (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was human IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetTPBG
IHC image of CSB-RA025141MA1HU diluted at 1 : 100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetTSLP
The Binding Activity of Human TSLP(R127A,R130A) with Anti-TSLP recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized human TSLP(R127A,R130A)(CSB-MP025141HU(M)) at 2 microg/mL can bind Anti-TSLP recombinant antibody. The EC50 is 52.55-58.67 ng/mL.
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetTSLP
The Binding Activity of TSPAN8 with Anti-TSPAN8 recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human TSPAN8 (CSB-MP025166HU) at 5 microg/mL can bind Anti-TSPAN8 recombinant antibody. The EC50 is 2.261-2.623 ng/mL. The VLPs (CSB-MP3838) is negative control.
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetTSPAN8
IHC image of CSB-RA025440MA1HU diluted at 1:300 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetUBE2C
IHC image of CSB-RA025857MA1HU diluted at 1:40 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Rabbit IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetVIM