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Product group Antibodies
OriGene
EGFR (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 13G8]AM00047BT-N
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
ReactivityHuman, Mouse
TargetEGFR
  • SizePrice
Detection of endogenous EGFR: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab EGFR-13G8 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2
Product group Antibodies
OriGene
EGFR (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 13G8]AM00047PU-N
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
ReactivityHuman, Mouse
TargetEGFR
  • SizePrice
ERBB2 Transactivation: Serum starved A431 cells were treated for 15 min as indicated. Whole cell lysates were separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab erbB2-7F8 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: Control Lane 2: PMA Lane 3: LPA Lane 4: Ceramide Lane 5: Bradykinin Lane 6: Bombesin
Product group Antibodies
OriGene
Her2 (ERBB2) pThr686 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 7F8]AM00048PU-N
ApplicationsWestern Blot
ReactivityCanine, Human, Mouse
TargetERBB2
  • SizePrice
Detection of endogenous erbB2 Whole cell extracts of serum starved, EGF-treated (15 min) tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab erbB2-24B5 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2A 549 Lane 3: SKOV3 Lane 4: OVCAR5 Lane 5: HaCaT Lane 6: PC3 Lane 7: HeLa Lane 8: HepG2
Product group Antibodies
OriGene
Her2 (ERBB2) (860-880) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 24B5]AM00049PU-N
ApplicationsWestern Blot
ReactivityCanine, Human, Mouse
TargetERBB2
  • SizePrice
Product group Antibodies
OriGene
Her2 (ERBB2) pTyr1112 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 19G5]AM00050BT-N
ApplicationsImmunoFluorescence, Western Blot
ReactivityCanine, Human, Mouse, Rat
TargetERBB2
  • SizePrice
Product group Antibodies
OriGene
Her2 (ERBB2) pTyr1112 Mouse Monoclonal Antibody [Clone ID: 19G5]AM00050FC-N
ApplicationsImmunoFluorescence
ReactivityCanine, Human, Mouse, Rat
TargetERBB2
  • SizePrice
ErbB2 activation: Serum starved A549 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab erbB2-19G5 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: Control Lane 2: 5 min EGF Lane 3: 15 min EGF Lane 4: 30 min EGF Lane 5: 1h EGF Lane 6; 2h EGF Lane 7: 4h EGF Lane 8: 8h EGF
Product group Antibodies
OriGene
Her2 (ERBB2) pTyr1112 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 19G5]AM00050PU-N
ApplicationsImmunoFluorescence, Western Blot
ReactivityCanine, Human, Mouse, Rat
TargetERBB2
  • SizePrice
Product group Antibodies
OriGene
Her2 (ERBB2) pSer1113 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 9E10]AM00051BT-N
ApplicationsImmunoFluorescence, Western Blot, ELISA
ReactivityHuman
TargetERBB2
  • SizePrice
erbB2 Transactivation Serum starved A431 cells were treated for 15min as indicated. Whole cell lysates were separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab erbB2 - 9E10 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: control; lane 2: TGFbeta; lane 3: Bradykinin; lane 4: Pervanadate; lane 5: Anisomycin; lane 6: PMA
Product group Antibodies
OriGene
Her2 (ERBB2) pSer1113 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 9E10]AM00051PU-N
ApplicationsImmunoFluorescence, Western Blot, ELISA
ReactivityHuman
TargetERBB2
  • SizePrice
Product group Antibodies
OriGene
Her2 (ERBB2) (1240-1260) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 19D2]AM00052BT-N
ApplicationsWestern Blot
ReactivityCanine, Human, Mouse
TargetERBB2
  • SizePrice
Detection of endogenous erbB2 Whole cell extracts of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab erbB2-19D2 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: MDA-MB 231; lane 2: MDA-MB 468; lane 3: MCF-7; lane 4: T47D; lane 5: SW480; lane 6: SW620
Product group Antibodies
OriGene
Her2 (ERBB2) (1240-1260) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 19D2]AM00052PU-N
ApplicationsWestern Blot
ReactivityCanine, Human, Mouse
TargetERBB2
  • SizePrice
erbB2 activation Serum starved OVCAR-5 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab erbB2 - 6G7 (0.5 ug/ ml) for 1h at RTand developed by ECL (exp. time: 30 sec). lane 1: control; lane 2: 5 min EGF; lane 3: 15 min EGF; lane 4: 30 min EGF; lane 5: 1h EGF;lane 6; 2h EGF; lane 7: 4h EGF; lane 8: 8h EGF
Product group Antibodies
OriGene
Her2 (ERBB2) pTyr1248 Mouse Monoclonal Antibody [Clone ID: 6G7]AM00053PU-N
ApplicationsWestern Blot
ReactivityHuman
TargetERBB2
  • SizePrice
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