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Mouse bone lysates probed with Proteasome 19S S5A Polyclonal Antibody, Unconjugated (bs-8322R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Mouse bone lysates probed with Proteasome 19S S5A Polyclonal Antibody, Unconjugated (bs-8322R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Mouse bone lysates probed with Proteasome 19S S5A Polyclonal Antibody, Unconjugated (bs-8322R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Proteasome 19S S5A Polyclonal Antibody

BS-8322R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetPSMD4
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Proteasome 19S S5A Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5710
  • Target name
    PSMD4
  • Target description
    proteasome 26S subunit ubiquitin receptor, non-ATPase 4
  • Target synonyms
    26S proteasome non-ATPase regulatory subunit 4; 26S proteasome regulatory subunit S5A; AF; AF-1; angiocidin; antisecretory factor 1; ASF; MCB1; multiubiquitin chain-binding protein; proteasome (prosome, macropain) 26S subunit, non-ATPase, 4; proteasome 26S subunit, non-ATPase 4; pUB-R5; Rpn10; S5A; S5a/antisecretory factor protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55036
  • Protein Name
    26S proteasome non-ATPase regulatory subunit 4
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PSMD4 using anti-PSMD4 antibody (A03544-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMD4 antigen affinity purified polyclonal antibody (Catalog # A03544-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMD4 at approximately 55 kDa. The expected band size for PSMD4 is at 41 kDa.
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