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Western Blot Positive WB detected in: HepG2 whole cell lysate, 293T whole cell lysate All lanes: PSMD4 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 41, 29 kDa Observed band size: 41 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, 293T whole cell lysate All lanes: PSMD4 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 41, 29 kDa Observed band size: 41 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, 293T whole cell lysate All lanes: PSMD4 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 41, 29 kDa Observed band size: 41 kDa

PSMD4 Antibody

CSB-PA018908HA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPSMD4
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Overview

  • Supplier
    Cusabio
  • Product Name
    PSMD4 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5710
  • Target name
    PSMD4
  • Target description
    proteasome 26S subunit ubiquitin receptor, non-ATPase 4
  • Target synonyms
    26S proteasome non-ATPase regulatory subunit 4; 26S proteasome regulatory subunit S5A; AF; AF-1; angiocidin; antisecretory factor 1; ASF; MCB1; multiubiquitin chain-binding protein; proteasome (prosome, macropain) 26S subunit, non-ATPase, 4; proteasome 26S subunit, non-ATPase 4; pUB-R5; Rpn10; S5A; S5a/antisecretory factor protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55036
  • Protein Name
    26S proteasome non-ATPase regulatory subunit 4
  • Scientific Description
    Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMD4 acts as an ubiquitin receptor subunit through ubiquitin-interacting motifs and selects ubiquitin-conjugates for destruction. Displays a preferred selectivity for longer polyubiquitin chains.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PSMD4 using anti-PSMD4 antibody (A03544-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMD4 antigen affinity purified polyclonal antibody (Catalog # A03544-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMD4 at approximately 55 kDa. The expected band size for PSMD4 is at 41 kDa.
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