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PSMD4 Antibody (YA2115, Rabbit)

HY-P82370
MedChem Express
TargetPSMD4
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    PSMD4 Antibody (YA2115, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID5710
  • Target name
    PSMD4
  • Target description
    proteasome 26S subunit ubiquitin receptor, non-ATPase 4
  • Target synonyms
    AF, AF-1, ASF, MCB1, Rpn10, S5A, pUB-R5, 26S proteasome non-ATPase regulatory subunit 4, 26S proteasome regulatory subunit S5A, S5a/antisecretory factor protein, angiocidin, antisecretory factor 1, multiubiquitin chain-binding protein, proteasome (prosome, macropain) 26S subunit, non-ATPase, 4, proteasome 26S subunit, non-ATPase 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    PSMD4 Antibody (YA2115) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PSMD4.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PSMD4 using anti-PSMD4 antibody (A03544-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMD4 antigen affinity purified polyclonal antibody (Catalog # A03544-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMD4 at approximately 55 kDa. The expected band size for PSMD4 is at 41 kDa.
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