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20S Immunoproteasome vs. 20S Constitutive Proteasome Activity: 20S Proteasome is most active against Suc-LLVY-AMC (SBB-PS0010), Ac-LLE-AMC (SBB-PS0006), and Ac-WLA-AMC (SBB-PS0008) substrates, representing physiologically relevant chymotrypsin-like, beta1c, and beta5c proteasome activity respectively.
Product group Proteins / Signaling Molecules
South Bay Bio
20S Proteasome (human) (untagged)SBB-PP0005
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Signal to Background: The signal to background ratio was determined by 100% hydrolysis of either 50nM or 500nM Ubiquitin-Rhodamine 110 to liberate the quenched conjugate. Assay Buffer: 50mM HEPES pH7.5, 100mM NaCl, 1mM TCEP, 0.1mg/ml BSA.
Product group Proteins / Signaling Molecules
South Bay Bio
Ubiquitin (human) (rec.) (Rhodamine 110)SBB-PS0001
Protein IDP0CG47
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Signal to Background. The signal to background ratio was determined by 100% hydrolysis of either 50nM or 500nM ISG15-Rhodamine 110 to liberate the quenched conjugate. Assay Buffer: 50mM HEPES pH7.5, 100mM NaCl, 1mM TCEP, 0.1mg/ml BSA.
Product group Proteins / Signaling Molecules
South Bay Bio
ISG15 (human) (rec.) (Rhodamine 110)SBB-PS0002
Protein IDP05161
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Signal to Background: The signal to background ratio was determined by 100% hydrolysis of 800nM, 400nM, 200nM, 100nM, 50nM NEDD8-Rhodamine 110 to liberate the quenched conjugate. Assay Buffer: 50mM HEPES pH7.5, 1mM TCEP, 0.1mg/ml BSA.
Product group Proteins / Signaling Molecules
South Bay Bio
NEDD8 (human) (rec.) (Rhodamine 110)SBB-PS0003
Protein IDQ15843
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Signal to Background: The signal to background ratio was determined by 100% hydrolysis of 200nM, 100nM, 50nM SUMO1-Rhodamine 110 to liberate the quenched conjugate. Assay Buffer: 50mM HEPES pH 7.5, 1mM TCEP, 0.1mg/ml BSA.
Product group Proteins / Signaling Molecules
South Bay Bio
SUMO1 (human) (rec.) (Rhodamine 110)SBB-PS0028
Protein IDP63165
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Signal to Background: The signal to background ratio was determined by 100% hydrolysis of 200nM, 100nM, 50nM SUMO2-Rhodamine 110 to liberate the quenched conjugate. Assay Buffer: 50mM HEPES pH 7.5, 1mM TCEP, 0.1mg/ml BSA.
Product group Proteins / Signaling Molecules
South Bay Bio
SUMO2 (human) (rec.) (Rhodamine 110)SBB-PS0029
Protein IDP61956
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Kinetic Activity: UCHL3 (SBB-DE0023) activity with Ubiquitin-AMC (SBB-PS0043) measured in the presence of 17.5nM and 0.1nM Ubiquitin Aldehyde (SBB-PS0031).
Product group Proteins / Signaling Molecules
South Bay Bio
Ubiquitin aldehyde (human) (rec.)SBB-PS0031
Protein IDP0CG47
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Kinetic Activity: UCHL3 (SBB-DE0023) activity with Ubiquitin-AMC (SBB-PS0043) measured in the presence of 12.5nM and 0.1nM Ubiquitin vinyl sulfone (SBB-PS0032).
Product group Proteins / Signaling Molecules
South Bay Bio
Ubiquitin vinyl sulfone (human) (rec.)SBB-PS0032
Protein IDP0CG47
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Kinetic Activity: UCHL3 (SBB-DE0023) activity with Ubiquitin-AMC (SBB-PS0043) measured in the presence of 15.0nM and 0.1nM Ubiquitin vinyl methyl ester (SBB-PS0033).
Product group Proteins / Signaling Molecules
South Bay Bio
Ubiquitin vinyl methyl ester (human) (rec.)SBB-PS0033
Protein IDP0CG47
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Kinetic Activity: UCHL3 (SBB-DE0023) activity with Ubiquitin-AMC (SBB-PS0043) measured in the presence of 15.0nM and 0.1nM Ubiquitin propargylamide (SBB-PS0034).
Product group Proteins / Signaling Molecules
South Bay Bio
Ubiquitin-propargylamide (human) (rec.)SBB-PS0034
Protein IDP0CG47
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Product group Proteins / Signaling Molecules
South Bay Bio
Ubiquitin (human) (rec.) (Europium-Cryptate)SBB-TR0014
Protein IDP0CG47
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Product group Proteins / Signaling Molecules
South Bay Bio
Ubiquitin (human) (rec.) (Cy5)SBB-TR0015
Protein IDP0CG47
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