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Western Blot analysis of Jurkat cell using PTGER1 Polyclonal Antibody at dilution of 1:500
Western Blot analysis of Jurkat cell using PTGER1 Polyclonal Antibody at dilution of 1:500
Western Blot analysis of Jurkat cell using PTGER1 Polyclonal Antibody at dilution of 1:500

PTGER1 Polyclonal Antibody

E-AB-15621
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    PTGER1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB
  • Certification
    Research Use Only
  • Concentration
    0.3mg/ml
  • Scientific Description
    The protein encoded by this gene is a member of the G protein-coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). Through a phosphatidylinositol-calcium second messenger system, G-Q proteins mediate this receptors activity. Knockout studies in mice suggested a role of this receptor in mediating algesia and in regulation of blood pressure. Studies in mice also suggested that this gene may mediate adrenocorticotropic hormone response to bacterial endotoxin.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PTGER1 using anti-PTGER1 antibody (PA1583). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGER1 antigen affinity purified polyclonal antibody (Catalog # PA1583) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGER1 at approximately 42 kDa. The expected band size for PTGER1 is at 42 kDa.
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