![Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PVRL4 antibody [HL2758] (GTX639621) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PVRL4 antibody [HL2758] (GTX639621) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639621/GTX639621_T-45278_20240126_WB_treatment_PNGaseF_24013018_397.webp)
Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PVRL4 antibody [HL2758] (GTX639621) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
PVRL4 antibody [HL2758]
GTX639621
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityHuman
TargetNECTIN4
Overview
- SupplierGeneTex
- Product NamePVRL4 antibody [HL2758]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, Western Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2758
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID81607
- Target nameNECTIN4
- Target descriptionnectin cell adhesion molecule 4
- Target synonymsEDSS1, LNIR, PRR4, PVRL4, nectin-4, nectin-4, Ig superfamily receptor LNIR, nectin 4, poliovirus receptor-related 4, poliovirus receptor-related protein 4
- HostRabbit
- IsotypeIgG
- Protein IDQ96NY8
- Protein NameNectin-4
- Scientific DescriptionThis gene encodes a member of the nectin family. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. Alternatively spliced transcript variants have been found but the full-length nature of the variant has not been determined.[provided by RefSeq, Jan 2011]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PVRL4 antibody [HL2758] (GTX639621) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PVRL4 antibody [HL2758] (GTX639621) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639621/GTX639621_45362_20240329_WB_TPM_watermark_24041019_473.webp)
![PVRL4 antibody [HL2758] (GTX639621) detects PVRL4 protein by flow cytometry analysis. Sample: non-fixed and non-permeabilized T-47D or MDA-MB-231 cells were stained with 5μg/ml GTX639621 (red) or a Rabbit monoclonal IgG isotype control (gray) in 1% FBS/ 5mM EDTA/PBS at 4oC for 1 hour. PVRL4 antibody [HL2758] (GTX639621) detects PVRL4 protein by flow cytometry analysis. Sample: non-fixed and non-permeabilized T-47D or MDA-MB-231 cells were stained with 5μg/ml GTX639621 (red) or a Rabbit monoclonal IgG isotype control (gray) in 1% FBS/ 5mM EDTA/PBS at 4oC for 1 hour.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639621/GTX639621_T-45278_20250214_FACS_25021923_858.webp)



![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PVRL4 antibody [HL3520] (GTX641417) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641417/GTX641417_T-45614_20241206_WB_TPM_watermark_24121100_253.webp)


