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Rabbit anti-BCAR3 Antibody, Affinity Purified

A301-671A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetBCAR3
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-BCAR3 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8412
  • Target name
    BCAR3
  • Target description
    BCAR3 adaptor protein, NSP family member
  • Target synonyms
    AND-34, MIG7, NSP2, SH2D3B, breast cancer anti-estrogen resistance protein 3, BCAR3, NSP family adaptor protein, SH2 domain-containing protein 3B, breast cancer anti-estrogen resistance 3, breast cancer antiestrogen resistance 3 protein, dJ1033H22.2 (breast cancer anti-estrogen resistance 3), epididymis secretory sperm binding protein, mig-7, migration inducting gene-7, novel SH2-containing protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75815
  • Protein Name
    Breast cancer anti-estrogen resistance protein 3
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BCAR3 using anti-BCAR3 antibody (PB9846). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HEPG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCAR3 antigen affinity purified polyclonal antibody (Catalog # PB9846) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCAR3 at approximately 93 kDa. The expected band size for BCAR3 is at 93 kDa.
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