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ProductsBack/Rabbit anti-Bcl6 Recombinant Monoclonal Antibody [BLR143H]

Rabbit anti-Bcl6 Recombinant Monoclonal Antibody [BLR143H]

A700-143
Bethyl Laboratories
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetBCL6
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-Bcl6 Recombinant Monoclonal Antibody [BLR143H]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    BLR143H
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID604
  • Target name
    BCL6
  • Target description
    BCL6 transcription repressor
  • Target synonyms
    BCL5, BCL6A, LAZ3, ZBTB27, ZNF51, B-cell lymphoma 6 protein, B cell CLL/lymphoma 6, B-cell lymphoma 5 protein, B-cell lymphoma 6 protein transcript, BCL-5, BCL-6, cys-his2 zinc finger transcription factor, lymphoma-associated zinc finger gene on chromosome 3, protein LAZ-3, zinc finger and BTB domain-containing protein 27, zinc finger protein 51, zinc finger transcription factor BCL6S
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP41182
  • Protein Name
    B-cell lymphoma 6 protein
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Bcl6 using anti-Bcl6 antibody (A00142-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji whole cell lysate, Lane 2: human MCF-7 whole cell lysate, Lane 3: human placenta tissue lysate, Lane 4: human A549 whole cell lysate, Lane 5: human Caco-2 whole cell lysate, Lane 6: human U2OS whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bcl6 antigen affinity purified polyclonal antibody (Catalog # A00142-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bcl6 at approximately 88KD. The expected band size for Bcl6 is at 79KD.
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