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ProductsBack/Rabbit anti-Bub1 Antibody, Affinity Purified

Rabbit anti-Bub1 Antibody, Affinity Purified

A300-373A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetBUB1
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-Bub1 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID699
  • Target name
    BUB1
  • Target description
    BUB1 mitotic checkpoint serine/threonine kinase
  • Target synonyms
    BUB1A, BUB1L, MCPH30, hBUB1, mitotic checkpoint serine/threonine-protein kinase BUB1, BUB1 budding uninhibited by benzimidazoles 1 homolog, budding uninhibited by benzimidazoles 1 homolog, mitotic spindle checkpoint kinase, putative serine/threonine-protein kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43683
  • Protein Name
    Mitotic checkpoint serine/threonine-protein kinase BUB1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BUB1 using anti-BUB1 antibody (A00776-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BUB1 antigen affinity purified polyclonal antibody (Catalog # A00776-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BUB1 at approximately 130 kDa. The expected band size for BUB1 is at 122 kDa.
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