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Rabbit anti Human C3c

RAHu/C3c
Nordic-MUbio
TargetC3
Product group Antibodies
1 ml
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Rabbit anti Human C3c
  • Delivery Days Customer
    7
  • Application Supplier Note
    Precipitation assays. In immunoelectrophoresis use 2 microl human plasma or equivalent against 120 microl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 microl antiserum in 3 mm diameter center well and 2 microl plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells. In single radial immunodiffusion use 1 percent antiserum in the gel.
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID718
  • Target name
    C3
  • Target description
    complement C3
  • Target synonyms
    acylation-stimulating protein cleavage product; AHUS5; ARMD9; ASP; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a; C3a anaphylatoxin; C3b; complement C3; complement component 3; complement component C3a; complement component C3b; CPAMD1; epididymis secretory sperm binding protein Li 62p; HEL-S-62p; prepro-C3
  • Host
    Rabbit
  • Protein IDP01024
  • Protein Name
    Complement C3
  • Source
    C3c is the major fragment resulting from the C3 cleavage by C3 convertase and factor i. It is composed of an intact beta chain bound to two fragments of the alpha chain. The protein is isolated and purified from pooled normal human serum by ...
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of C3 using anti-C3 antibody (A00168-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat plasma lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse plasma lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C3 antigen affinity purified polyclonal antibody (Catalog # A00168-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C3 at approximately 128KD/187KD. The expected band size for C3 is at 187KD.
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