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Complement C3 Antibody

LS-C835560
LifeSpan BioSciences
ApplicationsImmunoElectrophoresis, Other Application
Product group Antibodies
TargetC3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    Complement C3 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoElectrophoresis, Other Application
  • Applications Supplier
    DD (1:0), IE (1:0) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID718
  • Target name
    C3
  • Target description
    complement C3
  • Target synonyms
    acylation-stimulating protein cleavage product; AHUS5; ARMD9; ASP; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a; C3a anaphylatoxin; C3b; complement C3; complement component 3; complement component C3a; complement component C3b; CPAMD1; epididymis secretory sperm binding protein Li 62p; HEL-S-62p; prepro-C3
  • Host
    Goat
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of C3 using anti-C3 antibody (A00168-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat plasma lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse plasma lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C3 antigen affinity purified polyclonal antibody (Catalog # A00168-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C3 at approximately 128KD/187KD. The expected band size for C3 is at 187KD.
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