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Anti-NEP / DDR1 (Internal) Antibody

119-11294
RayBiotech
TargetDDR1
Product group Antibodies
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Rabbit Anti-Human NEP / DDR1 (Internal)
  • Delivery Days Customer
    4
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Gene ID780
  • Target name
    DDR1
  • Target description
    discoidin domain receptor tyrosine kinase 1
  • Target synonyms
    CAK; CD167; CD167 antigen-like family member A; cell adhesion kinase; DDR; EDDR1; epithelial discoidin domain-containing receptor 1; HGK2; mammary carcinoma kinase 10; MCK10; NEP; neuroepithelial tyrosine kinase; neurotrophic tyrosine kinase, receptor, type 4; NTRK4; protein-tyrosine kinase RTK-6; PTK3; PTK3A; PTK3A protein tyrosine kinase 3A; RTK6; TRKE; tyrosine kinase DDR; tyrosine-protein kinase CAK
  • Host
    Rabbit
  • Protein IDQ08345
  • Protein Name
    Epithelial discoidin domain-containing receptor 1
  • Scientific Description
    NEP / DDR1 Rabbit anti-Human Polyclonal (Internal) (Unconjugated) Antibody, (50 ug)
  • Storage Instruction
    -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DDR1 using anti-DDR1 antibody (A00905). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human A549 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDR1 antigen affinity purified polyclonal antibody (Catalog # A00905) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDR1 at approximately 101KD. The expected band size for DDR1 is at 101KD.
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