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ProductsBack/Rabbit anti Human Roundabout homolog 1, ROBO1

Rabbit anti Human Roundabout homolog 1, ROBO1

X2313P
Nordic-MUbio
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetROBO1

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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Rabbit anti Human Roundabout homolog 1, ROBO1
  • Delivery Days Customer
    7
  • Application Supplier Note
    Antibody can be used for Western blotting (1:400 starting dilution). Optimal concentration should be evaluated by serial dilutions.
  • Applications
    Western Blot
  • Applications Supplier
    Western Blotting;Western Blotting
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID6091
  • Target name
    ROBO1
  • Target description
    roundabout guidance receptor 1
  • Target synonyms
    CPHD8, DUTT1, NORS, NYS8, SAX3, roundabout homolog 1, deleted in U twenty twenty, roundabout, axon guidance receptor, homolog 1
  • Host
    Rabbit
  • Protein IDQ9Y6N7
  • Protein Name
    Roundabout homolog 1
  • Scientific Description
    H-Robo-1, Deleted in U twenty twenty
  • Shelf life instruction
    See expiration date on vial
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    Synthetic peptide derived from human ROBO1 protein
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ROBO1 using anti-ROBO1 antibody (A01530-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROBO1 antigen affinity purified polyclonal antibody (Catalog # A01530-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROBO1 at approximately 250 kDa. The expected band size for ROBO1 is at 181 kDa.
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Tissue: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; \nAntigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15min; Blocking buffer (normal goat serum 5%) at 37℃ for 20 min; \nIncubation: Anti-Robo Polyclonal Antibody, Unconjugated (bs-5794R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue) was used to stain the cell nuclei.
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References
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