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Rabbit anti-LRPPRC Antibody, Affinity Purified

A304-731A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetLRPPRC
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-LRPPRC Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10128
  • Target name
    LRPPRC
  • Target description
    leucine rich pentatricopeptide repeat containing
  • Target synonyms
    CLONE-23970, GP130, LRP130, LSFC, MC4DN5, leucine-rich PPR motif-containing protein, mitochondrial, 130 kDa leucine-rich protein, LRP 130, leucine-rich PPR-motif containing, mitochondrial leucine-rich PPR motif-containing protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42704
  • Protein Name
    Leucine-rich PPR motif-containing protein, mitochondrial
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate All lanes: LRPPRC antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 158 kDa Observed band size: 158 kDa
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Figure 1. Western blot analysis of LRPPRC using anti-LRPPRC antibody (A03264). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: monkey kidney tissue lysates, Lane 6: monkey heart tissue lysates, Lane 7: rat heart tissue lysates, Lane 8: rat skeletal muscle tissue lysates, Lane 9: rat kidney tissue lysates, Lane 10: rat liver tissue lysates, Lane 11: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRPPRC antigen affinity purified polyclonal antibody (Catalog # A03264) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRPPRC at approximately 157KD. The expected band size for LRPPRC is at 150-160KD.
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References
GeneTex
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