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Rabbit anti-NARFL Antibody, Affinity Purified

A305-769A
Bethyl Laboratories
ApplicationsImmunoPrecipitation
Product group Antibodies
TargetCIAO3
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-NARFL Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID64428
  • Target name
    CIAO3
  • Target description
    cytosolic iron-sulfur assembly component 3
  • Target synonyms
    cytosolic Fe-S cluster assembly factor NARFL; cytosolic iron-sulfur assembly component 3; HPRN; IOP1; iron-only hydrogenase-like protein 1; LET1 like/JFP15; LET1L; NAR1; NARFL; nuclear prelamin A recognition factor like; PRN; protein related to Narf
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H6Q4
  • Protein Name
    Cytosolic Fe-S cluster assembly factor NARFL
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NARFL/CIAO3 using anti-NARFL/CIAO3 antibody (A32387). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NARFL/CIAO3 antigen affinity purified polyclonal antibody (A32387) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NARFL/CIAO3 at approximately 53 kDa. The expected band size for NARFL/CIAO3 is at 53 kDa.
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