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Rabbit anti-NP95/UHRF1 IHC Antibody, Affinity Purified

IHC-00554
Bethyl Laboratories
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Primate
TargetUHRF1

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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-NP95/UHRF1 IHC Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    250 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID29128
  • Target name
    UHRF1
  • Target description
    ubiquitin like with PHD and ring finger domains 1
  • Target synonyms
    ICBP90, Np95, RNF106, TDRD22, hNP95, hUHRF1, huNp95, E3 ubiquitin-protein ligase UHRF1, RING finger protein 106, RING-type E3 ubiquitin transferase UHRF1, inverted CCAAT box binding protein 90, inverted CCAAT box-binding protein of 90 kDa, nuclear phosphoprotein 95, nuclear protein 95, nuclear zinc finger protein Np95, transcription factor ICBP90, ubiquitin-like PHD and RING finger domain-containing protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96T88
  • Protein Name
    E3 ubiquitin-protein ligase UHRF1
  • Reactivity
    Human, Monkey, Primate
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of UHRF1 using anti-UHRF1 antibody (A01156-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UHRF1 antigen affinity purified polyclonal antibody (Catalog # A01156-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UHRF1 at approximately 100 kDa. The expected band size for UHRF1 is at 90 kDa.
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