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ProductsBack/Rabbit anti-PD-L1 Recombinant Monoclonal Antibody [BLR226K]

Rabbit anti-PD-L1 Recombinant Monoclonal Antibody [BLR226K]

A700-226
Bethyl Laboratories
ApplicationsWestern Blot
Product group Antibodies
TargetCd274
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-PD-L1 Recombinant Monoclonal Antibody [BLR226K]
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    BLR226K
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID60533
  • Target name
    Cd274
  • Target description
    CD274 antigen
  • Target synonyms
    A530045L16Rik, B7h1, Pdcd1l1, Pdcd1lg1, Pdl1, programmed cell death 1 ligand 1, B7 homolog 1, PDCD1 ligand 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9EP73
  • Protein Name
    Programmed cell death 1 ligand 1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PD-L1/Cd274 using anti-PD-L1/Cd274 antibody (A00109-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PD-L1/Cd274 antigen affinity purified polyclonal antibody (Catalog # A00109-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PD-L1/Cd274 at approximately 40KD. The expected band size for PD-L1/Cd274 is at 40KD.
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