
Western blot showing GeneTexs Affinity Purified anti-HR23A antibody detects endogenous human HR23A. Reactivity is shown against HeLa nuclear extract (lane 1) and HeLa (lane 2), A431 (lane 3), Jurkat (lane 4) and 293 whole cell lysates (lane 5). Comparison to a molecular weight marker (at left) indicates a band of ~60 kDa corresponding to HR23A. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using HRP conjugated Rb-a-Goat IgG and chemilumi-nescence reagent with a 30-min exposure time. Other detection systems will yield similar results.
RAD23A antibody
GTX23836
Overview
- SupplierGeneTex
- Product NameRAD23A antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:2000. ELISA: 1:4000-1:16000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.2 mg/ml
- ConjugateUnconjugated
- Gene ID5886
- Target nameRAD23A
- Target descriptionRAD23 homolog A, nucleotide excision repair protein
- Target synonymsHHR23A; HR23A; RAD23, yeast homolog, A; UV excision repair protein RAD23 homolog A
- HostGoat
- IsotypeIgG
- Protein IDP54725
- Protein NameUV excision repair protein RAD23 homolog A
- Scientific DescriptionHHR23A was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203