ICC/IF analysis of GM0637 cells irradiated by X-rays (2 Gy) for 1 hrs using GTX00720 Rad51 antibody. Left : 1:10000 for 30 min at 37 degree C Right : 1:1000 for 30 min at 37 degree C Green : Primary antibody Blue : Hoechst 33342 Fixation : 4% PFA for 10 min Permeabilization : 0.5% Triton X-100 for 5 min
ICC/IF analysis of GM0637 cells irradiated by X-rays (2 Gy) for 1 hrs using GTX00720 Rad51 antibody. Left : 1:10000 for 30 min at 37 degree C Right : 1:1000 for 30 min at 37 degree C Green : Primary antibody Blue : Hoechst 33342 Fixation : 4% PFA for 10 min Permeabilization : 0.5% Triton X-100 for 5 min
Rad51 antibody
GTX00720
ApplicationsDot Blot, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityChicken, Hamster, Human, Mouse, Rodent, Rat, Xenopus
TargetRAD51
Overview
- SupplierGeneTex
- Product NameRad51 antibody
- Delivery Days Customer9
- ApplicationsDot Blot, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5888
- Target nameRAD51
- Target descriptionRAD51 recombinase
- Target synonymsBRCA1/BRCA2-containing complex, subunit 5; BRCC5; DNA repair protein RAD51 homolog 1; FANCR; HRAD51; HsRad51; HsT16930; MRMV2; RAD51 homolog A; RAD51A; RECA; RecA, E. coli, homolog of; RecA-like protein; recombination protein A
- HostRabbit
- IsotypeIgG
- Scientific DescriptionThe protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
- ReactivityChicken, Hamster, Human, Mouse, Rodent, Rat, Xenopus
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203