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Western Blot analysis of Hela cell using RAD51 Polyclonal Antibody at dilution of 1:200
Western Blot analysis of Hela cell using RAD51 Polyclonal Antibody at dilution of 1:200
Western Blot analysis of Hela cell using RAD51 Polyclonal Antibody at dilution of 1:200

RAD51 Polyclonal Antibody

E-AB-16750
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    RAD51 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.5mg/ml
  • Scientific Description
    The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, whicHuman, Mouseay be important for the cellular response to DNA damage.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Rad51 using anti-Rad51 antibody (A00088). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate, Lane 2: human A431 whole cell lysate, Lane 3: human 293T whole cell lysate, Lane 4: human K562 whole cell lysate, Lane 5: human Jurkat whole cell lysate, Lane 6: human A549 whole cell lysate, Lane 7: human Caco-2 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rad51 antigen affinity purified polyclonal antibody (Catalog # A00088) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rad51 at approximately 39KD. The expected band size for Rad51 is at 36KD.
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