RAS antibody [Y13-259]

GeneTex

RAS antibody [Y13-259]

9

Monoclonal Anti-vH RAS reacts with phosphorylated and non-phosphorylated forms of vH RAS and vK RAS p21s and reacts with the p21 transitional products of the H, K, and N ras human genes. It does not crossreact with Rap. This antibody neutralizes the activ

IF: Use at a concentration of 2.5 microg/ml. IHC-P: Use at a concentration of 5 microg/ml. Perform enzymatic antigen retrieval (0.05% saponin in water, 30 min. or 0.1% pepsin in 0.1 M HCl, 10-20 min. at room temperature) before commencing with IHC staining protocol. IHC-Fr: Use at a concentration of 5 microg/ml. IP: Use at an assay dependent dilution. WB: Use at a concentration of 2.5 microg/ml. Predicted molecular weight: 21 kDa. Optimal dilutions/concentrations should be determined by the end user.

ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

Y13-259

0.1 mg/ml

Unconjugated

HRAS

HRas proto-oncogene, GTPase

C-BAS/HAS; C-HA-RAS1; c-has/bas p21 protein; C-H-RAS; CTLO; GTP- and GDP-binding peptide B; GTPase HRas; HAMSV; Ha-Ras1 proto-oncoprotein; Harvey rat sarcoma viral oncogene homolog; Harvey rat sarcoma viral oncoprotein; HRAS1; H-RASIDX; p19 H-RasIDX protein; p21ras; Ras family small GTP binding protein H-Ras; RASH1; transformation gene: oncogene HAMSV; transforming protein p21; v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Rat

IgG

This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for

Human, Mouse, Rat

2°C to 8°C

12352203